Journal: PLoS ONE

Article Title: Insights from Characterizing Extinct Human Gut Microbiomes

doi: 10.1371/journal.pone.0051146

Figure Lengend Snippet: These data resulted from comparison of the 16S rRNA V3. Taxon distribution and cluster dendrogram were limited to phyla with a frequency of 5% or more.

Article Snippet: After removing primer sequences, barcoded data from pyrosequencing of 16S rRNA amplicons averaged 150 bases in length.

Techniques: Comparison

Journal: PLoS ONE

Article Title: Insights from Characterizing Extinct Human Gut Microbiomes

doi: 10.1371/journal.pone.0051146

Figure Lengend Snippet: Both Rio Zape samples assign partially to the rural African children source, with sample ZA04 also containing a predicted partial match to the modern non-human primate gut source. Caserones sample CA18 assigns almost entirely to the compost source with relatively high confidence; sample CA10 is predicted with low confidence to contain a small proportion of compost (See for variability in proportion estimates). The sources for the Hinds Cave samples were unrecognized given our training data, resulting in nearly complete assignments to the “Unknown” source. When extraction blanks were subjected to 60 cycles of 16S PCR, the amplified microbial community signature assigns to either a skin community or unknown community.

Article Snippet: After removing primer sequences, barcoded data from pyrosequencing of 16S rRNA amplicons averaged 150 bases in length.

Techniques: Extraction, Amplification